{
  "tool": "openbiota",
  "generated": "2026-09-04T04:24:58-0700",
  "reference_fingerprint": "b795fba24e3be8a2",
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          "panel": "ipa",
          "gene": "fldH",
          "truly_present": true,
          "true_copies_per_100": 8.3333,
          "detected": true,
          "reported_fragments": 84,
          "reported_copies_per_100": 10.781,
          "mean_identity": 89.2,
          "classification": "TP",
          "true_copies_residue_filtered": 8.3333,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "pcresol:HPDB",
          "panel": "pcresol",
          "gene": "hpdB",
          "truly_present": true,
          "true_copies_per_100": 25.0,
          "detected": true,
          "reported_fragments": 217,
          "reported_copies_per_100": 10.5915,
          "mean_identity": 92.0,
          "classification": "TP",
          "true_copies_residue_filtered": 25.0,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "propionate:PCT",
          "panel": "propionate",
          "gene": "pct",
          "truly_present": true,
          "true_copies_per_100": 8.3333,
          "detected": true,
          "reported_fragments": 257,
          "reported_copies_per_100": 20.1993,
          "mean_identity": 83.4,
          "classification": "TP",
          "true_copies_residue_filtered": 8.3333,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "propionate:PDUP",
          "panel": "propionate",
          "gene": "pduP",
          "truly_present": true,
          "true_copies_per_100": 8.3333,
          "detected": true,
          "reported_fragments": 218,
          "reported_copies_per_100": 19.3429,
          "mean_identity": 81.0,
          "classification": "TP",
          "true_copies_residue_filtered": 8.3333,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "propionate:LCDA",
          "panel": "propionate",
          "gene": "lcdA",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": true,
          "reported_fragments": 144,
          "reported_copies_per_100": 20.2498,
          "mean_identity": 66.2,
          "classification": "FP",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "urda:URDA",
          "panel": "urda",
          "gene": "urdA",
          "truly_present": true,
          "true_copies_per_100": 75.0,
          "detected": true,
          "reported_fragments": 1953,
          "reported_copies_per_100": 126.7962,
          "mean_identity": 87.7,
          "classification": "TP",
          "true_copies_residue_filtered": 29.1667,
          "reported_copies_residue_consistent": 50.735
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      ]
    },
    {
      "name": "negative",
      "description": "6 abundant gut organisms with no known carriage of the target genes. Gene presence is verified from each proteome, so anything reported for an absent gene is a false positive. This is the experiment that measures the false-positive rate directly.",
      "n_read_pairs": 3000000,
      "community": [
        {
          "label": "btheta",
          "organism": "Bacteroides thetaiotaomicron",
          "accession": "GCF_014131755.1",
          "cell_fraction": 0.166667,
          "genome_size": 6304193
        },
        {
          "label": "pcopri",
          "organism": "Segatella copri DSM 18205",
          "accession": "GCF_020735445.1",
          "cell_fraction": 0.166667,
          "genome_size": 3551614
        },
        {
          "label": "amuciniphila",
          "organism": "Akkermansia muciniphila",
          "accession": "GCF_009731575.1",
          "cell_fraction": 0.166667,
          "genome_size": 2878459
        },
        {
          "label": "badolescentis",
          "organism": "Bifidobacterium adolescentis ATCC 15703",
          "accession": "GCF_000010425.1",
          "cell_fraction": 0.166667,
          "genome_size": 2089645
        },
        {
          "label": "blongum",
          "organism": "Bifidobacterium longum subsp. longum JCM 1217",
          "accession": "GCF_000196555.1",
          "cell_fraction": 0.166667,
          "genome_size": 2385164
        },
        {
          "label": "sthermophilus",
          "organism": "Streptococcus thermophilus",
          "accession": "GCF_903886475.1",
          "cell_fraction": 0.166667,
          "genome_size": 1791634
        }
      ],
      "rpob_fragments": 3589,
      "confusion": {
        "TP": 3,
        "FP": 1,
        "FN": 0,
        "TN": 15
      },
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        {
          "entry_key": "bai:BAICD",
          "panel": "bai",
          "gene": "baiCD",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
          "mean_identity": null,
          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "bai:BAIH",
          "panel": "bai",
          "gene": "baiH",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
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          "mean_identity": null,
          "classification": "TN",
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          "reported_copies_residue_consistent": null
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          "panel": "bai",
          "gene": "baiB",
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          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
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          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
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        {
          "entry_key": "bai:BAIF",
          "panel": "bai",
          "gene": "baiF",
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          "reported_fragments": 0,
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          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "bai:BAIE",
          "panel": "bai",
          "gene": "baiE",
          "truly_present": false,
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          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
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          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "bai:BAIA",
          "panel": "bai",
          "gene": "baiA",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
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          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
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        {
          "entry_key": "bsh:BSH",
          "panel": "bsh",
          "gene": "bsh",
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          "true_copies_per_100": 33.3333,
          "detected": true,
          "reported_fragments": 397,
          "reported_copies_per_100": 44.214,
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          "classification": "TP",
          "true_copies_residue_filtered": 33.3333,
          "reported_copies_residue_consistent": null
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        {
          "entry_key": "butyrate:BUT",
          "panel": "butyrate",
          "gene": "but",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
          "mean_identity": null,
          "classification": "TN",
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          "reported_copies_residue_consistent": null
        },
        {
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          "panel": "butyrate",
          "gene": "buk",
          "truly_present": true,
          "true_copies_per_100": 16.6667,
          "detected": true,
          "reported_fragments": 188,
          "reported_copies_per_100": 18.776,
          "mean_identity": 99.7,
          "classification": "TP",
          "true_copies_residue_filtered": 16.6667,
          "reported_copies_residue_consistent": null
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        {
          "entry_key": "cutc:CUTC",
          "panel": "cutc",
          "gene": "cutC",
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          "true_copies_per_100": 0.0,
          "detected": false,
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          "reported_copies_per_100": 0.0,
          "mean_identity": null,
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          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
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          "panel": "cutc",
          "gene": "cntA",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
          "mean_identity": null,
          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "indole:TNAA",
          "panel": "indole",
          "gene": "tnaA",
          "truly_present": true,
          "true_copies_per_100": 33.3333,
          "detected": true,
          "reported_fragments": 301,
          "reported_copies_per_100": 23.1778,
          "mean_identity": 99.4,
          "classification": "TP",
          "true_copies_residue_filtered": 33.3333,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "ipa:FLDBC",
          "panel": "ipa",
          "gene": "fldBC",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
          "mean_identity": null,
          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "ipa:FLDH",
          "panel": "ipa",
          "gene": "fldH",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
          "mean_identity": null,
          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "pcresol:HPDB",
          "panel": "pcresol",
          "gene": "hpdB",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
          "mean_identity": null,
          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "propionate:PCT",
          "panel": "propionate",
          "gene": "pct",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
          "mean_identity": null,
          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "propionate:PDUP",
          "panel": "propionate",
          "gene": "pduP",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
          "mean_identity": null,
          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "propionate:LCDA",
          "panel": "propionate",
          "gene": "lcdA",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
          "mean_identity": null,
          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
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        {
          "entry_key": "urda:URDA",
          "panel": "urda",
          "gene": "urdA",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": true,
          "reported_fragments": 52,
          "reported_copies_per_100": 2.9114,
          "mean_identity": 68.3,
          "classification": "FP",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": 2.6665
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      ]
    },
    {
      "name": "spike_0p05",
      "description": "Streptococcus pasteurianus spiked at 5.00% of cells into a background of non-carriers. Finds the abundance at which detection fails.",
      "n_read_pairs": 3000000,
      "community": [
        {
          "label": "btheta",
          "organism": "Bacteroides thetaiotaomicron",
          "accession": "GCF_014131755.1",
          "cell_fraction": 0.158333,
          "genome_size": 6304193
        },
        {
          "label": "pcopri",
          "organism": "Segatella copri DSM 18205",
          "accession": "GCF_020735445.1",
          "cell_fraction": 0.158333,
          "genome_size": 3551614
        },
        {
          "label": "amuciniphila",
          "organism": "Akkermansia muciniphila",
          "accession": "GCF_009731575.1",
          "cell_fraction": 0.158333,
          "genome_size": 2878459
        },
        {
          "label": "badolescentis",
          "organism": "Bifidobacterium adolescentis ATCC 15703",
          "accession": "GCF_000010425.1",
          "cell_fraction": 0.158333,
          "genome_size": 2089645
        },
        {
          "label": "blongum",
          "organism": "Bifidobacterium longum subsp. longum JCM 1217",
          "accession": "GCF_000196555.1",
          "cell_fraction": 0.158333,
          "genome_size": 2385164
        },
        {
          "label": "sthermophilus",
          "organism": "Streptococcus thermophilus",
          "accession": "GCF_903886475.1",
          "cell_fraction": 0.158333,
          "genome_size": 1791634
        },
        {
          "label": "spasteurianus",
          "organism": "Streptococcus pasteurianus",
          "accession": "GCF_055380925.1",
          "cell_fraction": 0.05,
          "genome_size": 2177854
        }
      ],
      "rpob_fragments": 3706,
      "confusion": {
        "TP": 4,
        "FP": 1,
        "FN": 0,
        "TN": 14
      },
      "genes": [
        {
          "entry_key": "bai:BAICD",
          "panel": "bai",
          "gene": "baiCD",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
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          "mean_identity": null,
          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
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        {
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          "panel": "bai",
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          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
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          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
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          "panel": "bai",
          "gene": "baiB",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
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          "reported_copies_residue_consistent": null
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        {
          "entry_key": "bai:BAIF",
          "panel": "bai",
          "gene": "baiF",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
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          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
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        {
          "entry_key": "bai:BAIE",
          "panel": "bai",
          "gene": "baiE",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
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          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "bai:BAIA",
          "panel": "bai",
          "gene": "baiA",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
          "mean_identity": null,
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          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
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        {
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          "panel": "bsh",
          "gene": "bsh",
          "truly_present": true,
          "true_copies_per_100": 36.6667,
          "detected": true,
          "reported_fragments": 464,
          "reported_copies_per_100": 50.0444,
          "mean_identity": 98.3,
          "classification": "TP",
          "true_copies_residue_filtered": 36.6667,
          "reported_copies_residue_consistent": null
        },
        {
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          "panel": "butyrate",
          "gene": "but",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
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          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
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          "panel": "butyrate",
          "gene": "buk",
          "truly_present": true,
          "true_copies_per_100": 15.8333,
          "detected": true,
          "reported_fragments": 202,
          "reported_copies_per_100": 19.5373,
          "mean_identity": 99.7,
          "classification": "TP",
          "true_copies_residue_filtered": 15.8333,
          "reported_copies_residue_consistent": null
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        {
          "entry_key": "cutc:CUTC",
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          "gene": "cutC",
          "truly_present": false,
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          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
          "mean_identity": null,
          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "cutc:CNTA",
          "panel": "cutc",
          "gene": "cntA",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
          "mean_identity": null,
          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "indole:TNAA",
          "panel": "indole",
          "gene": "tnaA",
          "truly_present": true,
          "true_copies_per_100": 31.6667,
          "detected": true,
          "reported_fragments": 253,
          "reported_copies_per_100": 18.8666,
          "mean_identity": 99.3,
          "classification": "TP",
          "true_copies_residue_filtered": 31.6667,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "ipa:FLDBC",
          "panel": "ipa",
          "gene": "fldBC",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
          "mean_identity": null,
          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "ipa:FLDH",
          "panel": "ipa",
          "gene": "fldH",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": true,
          "reported_fragments": 1,
          "reported_copies_per_100": 0.1072,
          "mean_identity": 72.4,
          "classification": "FP",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "pcresol:HPDB",
          "panel": "pcresol",
          "gene": "hpdB",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
          "mean_identity": null,
          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "propionate:PCT",
          "panel": "propionate",
          "gene": "pct",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
          "mean_identity": null,
          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
        {
          "entry_key": "propionate:PDUP",
          "panel": "propionate",
          "gene": "pduP",
          "truly_present": false,
          "true_copies_per_100": 0.0,
          "detected": false,
          "reported_fragments": 0,
          "reported_copies_per_100": 0.0,
          "mean_identity": null,
          "classification": "TN",
          "true_copies_residue_filtered": 0.0,
          "reported_copies_residue_consistent": null
        },
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